I obtained a BSc in Honours Co-op Applied Chemistry from the University of Waterloo,
and a PhD in the NMR group in the Biochemistry Department at McGill.
I was studying PBX, a homeodomain protein, which in a mutated form is
implicated in pre B-cell leukemia. The project has involved determining the
structure of the free PBX homeodomain, the homeodomain bound to DNA, and a
trimeric complex of the homeodomain, DNA, and a peptide that increases the
affinity of the homeodomain for its DNA recognition site. Looking at the
changes that take place when PBX binds to DNA and its peptide binding
partner aid in understanding its role in transcription, and how mutations can
affect its functioning correctly. A variety of NMR techniques, including the
use of filtered experiments to observe one component of the complex, and the
measurement of residual dipolar couplings to orient the molecules relative
to one another have been employed in my research.
Currently, NMR Facility Manager of Quebec/Eastern Canada NMR Centre.